Human A-kinase anchor protein 4(AKAP4) ELISA kit

Human A-kinase anchor protein 4(AKAP4) ELISA kit

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Mfr.No.

CSB-EL001526HU

Alternative Names

A kinase (PRKA) anchor protein 4, RP13-377G1__B.1, AKAP82, CT99, FSC1, HI, hAKAP82, p82, A-kinase anchor protein 4, A-kinase anchor protein 82 kDa, cancer/testis antigen 99, protein kinase A anchoring protein 4, testis-specific gene HI

Reactivity

Human

Detection Range

25 pg/mL-1600 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human PGRMC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	96-106
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PGRMC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-98
1:2	Average %	96
	Range %	90-102
1:4	Average %	93
	Range %	85-99
1:8	Average %	89
	Range %	80-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	2.124	2.198	2.161	2.046
1500	1.409	1.415	1.412	1.297
750	0.923	0.951	0.937	0.822
375	0.588	0.590	0.589	0.474
187.5	0.390	0.384	0.387	0.272
94	0.274	0.286	0.280	0.165
47	0.197	0.190	0.194	0.079
0	0.114	0.116	0.115	?

Species

Homo sapiens

Sensitivity

6.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q5JQC9
Antigen	A kinase (PRKA) anchor protein 4

Applications

ELISA

Research Area

AKAP4

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501111	CSB-EL001526HU	96T	$695	Add to Cart
BHE10501111	CSB-EL001526HU	5x96T	$3336	Add to Cart
BHE10501111	CSB-EL001526HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.