Human A-kinase Anchor Protein 12

Mfr.No.

E4100hu

Sensitivity

7.59ng/L

Detection Range

15-3800ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human A-kinase Anchor Protein 12 (also known as AKAP12) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

AKAP 12;AKAP 250;AKAP12;AKAP-12;A-kinase anchor protein 12;A-kinase anchor protein 250 kDa;Gravin;Myasthenia gravis autoantigen

Molecular Weight

191482

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E4100hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1789.9	44.73	2.5
Intra-Assay	2	18	606.6	30.58	5
Intra-Assay	3	18	1098.7	52.43	4.8

Background

Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).

Protocol

These standard curves of E4100hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	1.966	2.021	1.97
	2.076
1000ng/L	1.101	1.008	0.957
	0.915
500ng/L	0.612	0.593	0.542
	0.574
250ng/L	0.247	0.211	0.161
	0.175
125ng/L	0.158	0.125	0.074
	0.092
0ng/L	0.045	0.051	0
	0.057

Gene ID	9590
UniProt ID	Q02952

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105506	E4100hu	48T	$320		Add to Cart
BHE12105506	E4100hu	96T	$458		Add to Cart

Human A-kinase Anchor Protein 12 - AKAP12 ELISA Kit

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