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Home / ELISA Kit

Human A Disintegrin And Metalloprotease 8 (ADAM8) ELISA Kit

SKU : BHE13703132

ADAM8 is expressed in macrophages, granulocytes, monocytes and B cells. It is an osteoclast stimulating factor. ADAM8 may play a role in neuron-glia interactions during neurodegeneration. It may also mediate extravasation of leukocytes either by degrading the vascular basement membrane or by liberating active molecules from their precursors harbored in the cell surface of leukocytes and endothelial cells.
Human ADAM8 precursor consists of 824 amino acid (aa) residues consisting of a 16 aa signal peptide, a 637 aa ectodomain including pro-, metalloprotease, disintegrin-like and cysteine-rich regions, a 25 aa transmembrane domain, and a 146 aa cytoplasmic domain). ADAM8 naturally occurs as both a membrane and a soluble form.

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Mfr.No.	KTE60916
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human A Disintegrin And Metalloprotease 8 (ADAM8) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM8.
Alternative Names	ADAM8; CD156; MGC134985; MS2; a disintegrin and metalloproteinase domain 8; cell surface antigen MS2; disintegrin and metalloproteinase domain-containing protein 8; human leukocyte differentiation antigen
Detection Method	Colorimetric
Kit Components	Human A Disintegrin And Metalloprotease 8 microplate Human A Disintegrin And Metalloprotease 8 standard Human A Disintegrin And Metalloprotease 8 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human A Disintegrin And Metalloprotease 8 (ADAM8) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADAM8. No significant cross-reactivity or interference between Human ADAM8 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	ADAM8 is expressed in macrophages, granulocytes, monocytes and B cells. It is an osteoclast stimulating factor. ADAM8 may play a role in neuron-glia interactions during neurodegeneration. It may also mediate extravasation of leukocytes either by degrading the vascular basement membrane or by liberating active molecules from their precursors harbored in the cell surface of leukocytes and endothelial cells. Human ADAM8 precursor consists of 824 amino acid (aa) residues consisting of a 16 aa signal peptide, a 637 aa ectodomain including pro-, metalloprotease, disintegrin-like and cysteine-rich regions, a 25 aa transmembrane domain, and a 146 aa cytoplasmic domain). ADAM8 naturally occurs as both a membrane and a soluble form.
Notes	This Human A Disintegrin And Metalloprotease 8 (ADAM8) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM8 in samples. An antibody specific for ADAM8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAM8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	101

Applications	ELISA
Applications Notes	This Human A Disintegrin And Metalloprotease 8 (ADAM8) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM8 in samples. An antibody specific for ADAM8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAM8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703132	KTE60916	48 T	$339		Add to Cart
BHE13703132	KTE60916	96 T	$589		Add to Cart
BHE13703132	KTE60916	96 Tx5	$2629		Add to Cart
BHE13703132	KTE60916	96 Tx50	$24569		Add to Cart

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