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Home / ELISA Kit

Human A Disintegrin And Metalloprotease 15 (ADAM15) ELISA Kit

SKU : BHE13703127

The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. ADAM15 contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as an EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.

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Mfr.No.	KTE60911
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human A Disintegrin And Metalloprotease 15 (ADAM15) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM15.
Alternative Names	ADAM15; RP11-540D14.1; MDC15; a disintegrin and metalloproteinase domain 15; a disintegrin and metalloproteinase domain 15 (metargidin); metalloprotease RGD disintegrin protein; metalloproteinase-like; disinte
Detection Method	Colorimetric
Kit Components	Human A Disintegrin And Metalloprotease 15 microplate Human A Disintegrin And Metalloprotease 15 standard Human A Disintegrin And Metalloprotease 15 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human A Disintegrin And Metalloprotease 15 (ADAM15) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADAM15. No significant cross-reactivity or interference between Human ADAM15 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. ADAM15 contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as an EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.
Notes	This Human A Disintegrin And Metalloprotease 15 (ADAM15) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM15 in samples. An antibody specific for ADAM15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAM15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM15 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM15 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	8751

Applications	ELISA
Applications Notes	This Human A Disintegrin And Metalloprotease 15 (ADAM15) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM15 in samples. An antibody specific for ADAM15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAM15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM15 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM15 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703127	KTE60911	48 T	$339		Add to Cart
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