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Home / ELISA Kit

Human A Disintegrin And Metalloprotease 12 (ADAM12) ELISA Kit

SKU : BHE13703126

ADAM12 encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. Similarly to myogenin, a marker of early muscle differentiation, mouse meltrin-alpha is expressed in neonatal muscle and bone, and its expression increases dramatically in response to the induction of differentiation. Immunocytochemical localization and functional expression studies suggested that meltrin-alpha may be involved in myotube formation.

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Mfr.No.	KTE60910
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human A Disintegrin And Metalloprotease 12 (ADAM12) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM12.
Alternative Names	ADAM12; RP11-295J3.5; MCMP; MCMPMltna; MLTN; MLTNA; a disintegrin and metalloproteinase domain 12 (meltrin alpha); meltrin alpha
Detection Method	Colorimetric
Kit Components	Human A Disintegrin And Metalloprotease 12 microplate Human A Disintegrin And Metalloprotease 12 standard Human A Disintegrin And Metalloprotease 12 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human A Disintegrin And Metalloprotease 12 (ADAM12) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADAM12. No significant cross-reactivity or interference between Human ADAM12 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	ADAM12 encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. Similarly to myogenin, a marker of early muscle differentiation, mouse meltrin-alpha is expressed in neonatal muscle and bone, and its expression increases dramatically in response to the induction of differentiation. Immunocytochemical localization and functional expression studies suggested that meltrin-alpha may be involved in myotube formation.
Notes	This Human A Disintegrin And Metalloprotease 12 (ADAM12) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM12 in samples. An antibody specific for ADAM12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAM12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	8038

Applications	ELISA
Applications Notes	This Human A Disintegrin And Metalloprotease 12 (ADAM12) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM12 in samples. An antibody specific for ADAM12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAM12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703126	KTE60910	48 T	$359		Add to Cart
BHE13703126	KTE60910	96 T	$619		Add to Cart
BHE13703126	KTE60910	96 Tx5	$2809		Add to Cart
BHE13703126	KTE60910	96 Tx50	$26209		Add to Cart

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