Human A Disintegrin And Metalloprotease 12,ADAM12 ELISA Kit

Human A Disintegrin And Metalloprotease 12,ADAM12 ELISA Kit

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Mfr.No.

CSB-E13163h

Sensitivity

7.8 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

ADAM metallopeptidase domain 12, RP11-295J3.5, MCMP, MCMPMltna, MLTN, MLTNA, a disintegrin and metalloproteinase domain 12 (meltrin alpha), meltrin alpha

Recovery

The recovery of human BMP-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-101
EDTA plasma (n=4)	92	88-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human BMP-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-99
1:2	Average %	95
	Range %	89-102
1:4	Average %	91
	Range %	84-105
1:8	Average %	92
	Range %	86-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.280	2.217	2.249	2.154
1000	1.925	1.987	1.956	1.861
500	1.515	1.624	1.570	1.475
250	1.048	1.008	1.028	0.933
125	0.596	0.583	0.590	0.495
62.5	0.374	0.355	0.365	0.270
31.25	0.241	0.252	0.247	0.152
0	0.096	0.093	0.095

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O43184

Applications	ELISA
Research Area	ADAM12

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10501071	CSB-E13163h	10x96T	$7932.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.