Human A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit

Human A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit

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Mfr.No.

CSB-E11172h

Alternative Names

ADAM metallopeptidase domain 10, AD10, CD156c, HsT18717, MADM, kuz, a disintegrin and metalloprotease domain 10, a disintegrin and metalloproteinase domain 10, kuzbanian

Reactivity

Human

Detection Range

7.8 pg/mL-500 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human PRDX1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-95
EDTA plasma (n=4)	100	97-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PRDX1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-91
1:2	Average %	92
	Range %	87-97
1:4	Average %	96
	Range %	93-99
1:8	Average %	97
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.462	2.497	2.480	2.295
100	1.726	1.736	1.731	1.546
50	1.121	1.164	1.143	0.958
25	0.683	0.698	0.691	0.506
12.5	0.475	0.498	0.487	0.302
6.25	0.351	0.367	0.359	0.174
3.12	0.297	0.287	0.292	0.107
0	0.186	0.183	0.185

Species

Homo sapiens

Sensitivity

1.95 pg/mL

Limit Of Detection

450 nm

UniProt ID	O14672
Antigen	ADAM metallopeptidase domain 10

Applications

ELISA

Research Area

ADAM10

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501070	CSB-E11172h	96T	$695	Add to Cart
BHE10501070	CSB-E11172h	5x96T	$3336	Add to Cart
BHE10501070	CSB-E11172h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.