Human 7-Dehydrocholesterol Reductase

Mfr.No.

E4885Hu

Sensitivity

9.28ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

7-dehydrocholesterol reductase;7-DHC reductase;Delta7-sterol reductase;DHCR 7;DHCR7;Sterol Delta(7-reductase;Sterol reductase SR-2

Molecular Weight

54489

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E4885Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	710.1	28.74	4
Intra-Assay	2	18	1519.8	55.85	3.7
Intra-Assay	3	18	354.1	17.22	4.9

Background

7-dehydrocholesterol reductase of the cholesterol biosynthetic pathway reducing the C7-C8 double bond of cholesta-5,7-dien-3beta-ol (7-dehydrocholesterol/7-DHC) and cholesta-5,7,24-trien-3beta-ol, two intermidiates in that pathway.

Protocol

These standard curves of E4885Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.159	2.195	2.164
	2.23
1200ng/L	1.445	1.423	1.392
	1.401
600ng/L	0.635	0.667	0.636
	0.698
300ng/L	0.361	0.336	0.305
	0.31
150ng/L	0.176	0.151	0.121
	0.126
0ng/L	0.041	0.03	0
	0.02

Gene ID	1717
UniProt ID	Q9UBM7

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106158	E4885Hu	48T	$320		Add to Cart
BHE12106158	E4885Hu	96T	$458		Add to Cart

Human 7-Dehydrocholesterol Reductase - DHCR7 ELISA Kit

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