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Home / ELISA Kit

Human 6-phosphofructokinase, muscle type (PFKM) ELISA Kit

SKU : BHE13703459

Phosphofructokinase-1 (PFK-1) is the most important regulatory enzyme (EC 2.7.1.11) of glycolysis. It is an allosteric enzyme made of 4 subunits and controlled by several activators and inhibitors. PFK-1 catalyzes one of the important "committed" steps of glycolysis, the conversion of fructose 6-phosphate and ATP to fructose 1,6-bisphosphate and ADP.
PFK1 is allosterically inhibited by ATP and citrate (from the citric acid cycle) and its product. It is also inhibited by low pH to prevent the accumulation of hydrogen ions in muscle. The enzyme has two sites with different affinities for ATP which is both a substrate and an inhibitor.PFK1 is allosterically activated by a high concentration of AMP, but the most potent activator is fructose 2,6-bisphosphate, which is also produced from fructose-6-phosphate by PFK2.

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Mfr.No.	KTE61243
Description	This Human 6-phosphofructokinase, muscle type (PFKM) ELISA Kit employs a two-site sandwich ELISA to quantitate PFKM.
Alternative Names	PFKM; GSD7; MGC8699; PFK-1; PFK1; PFKA; PFKX; 6-phosphofructo-1-kinase; phosphofructo-1-kinase isozyme A; phosphofructokinase; polypeptide X; phosphohexokinase
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human 6-phosphofructokinase, muscle type microplate Human 6-phosphofructokinase, muscle type standard Human 6-phosphofructokinase, muscle type detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human 6-phosphofructokinase, muscle type (PFKM) ELISA Kit has high sensitivity and excellent specificity for detection of Human PFKM. No significant cross-reactivity or interference between Human PFKM and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Phosphofructokinase-1 (PFK-1) is the most important regulatory enzyme (EC 2.7.1.11) of glycolysis. It is an allosteric enzyme made of 4 subunits and controlled by several activators and inhibitors. PFK-1 catalyzes one of the important "committed" steps of glycolysis, the conversion of fructose 6-phosphate and ATP to fructose 1,6-bisphosphate and ADP. PFK1 is allosterically inhibited by ATP and citrate (from the citric acid cycle) and its product. It is also inhibited by low pH to prevent the accumulation of hydrogen ions in muscle. The enzyme has two sites with different affinities for ATP which is both a substrate and an inhibitor.PFK1 is allosterically activated by a high concentration of AMP, but the most potent activator is fructose 2,6-bisphosphate, which is also produced from fructose-6-phosphate by PFK2.
Notes	This Human 6-phosphofructokinase, muscle type (PFKM) ELISA Kit employs a two-site sandwich ELISA to quantitate PFKM in samples. An antibody specific for PFKM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPFKM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PFKM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PFKM bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	5213
Antigen	PFKM
Tag	PFKM
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human 6-phosphofructokinase, muscle type (PFKM) ELISA Kit employs a two-site sandwich ELISA to quantitate PFKM in samples. An antibody specific for PFKM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPFKM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PFKM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PFKM bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703459	KTE61243	48 T	$339		Add to Cart
BHE13703459	KTE61243	96 T	$589		Add to Cart
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BHE13703459	KTE61243	96 Tx50	$24569		Add to Cart

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