Human 6-keto-prostaglandin F1a,6-K-PGF1a ELISA Kit

Mfr.No.

CSB-E09420h

Alternative Names

6-keto-prostaglandin F1a,6-K-PGF1a

Reactivity

Human

Detection Range

50 pg/mL-800 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of rat VDR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	81-89
EDTA plasma (n=4)	95	91-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat VDR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-93
1:2	Average %	101
	Range %	98-105
1:4	Average %	96
	Range %	93-99
1:8	Average %	105
	Range %	101-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

50 pg/mL

Limit Of Detection

450 nm

Antigen	6-keto-prostaglandin F1a,6-K-PGF1a

Applications

ELISA

Research Area

6ketoPGF1a

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501009	CSB-E09420h	96T	$595	Add to Cart
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BHE10501009	CSB-E09420h	10x96T	$5652.50	Add to Cart