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Home / ELISA Kit

Human 5-oxoprolinase - OPLAH ELISA Kit

SKU : BHE12115327

Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.

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Bioassay Technology Laboratory

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+1 866.986.9598

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Mfr.No.

E6640Hu

Sensitivity

0.016ng/ml

Detection Range

0.05-15ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

5-Opase;5-oxo-L-prolinase;5-oxoprolinase;OPLA;OPLAH;OPLAHD;Pyroglutamase

Molecular Weight

137457

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6640Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.16	0.12	2.98
Intra-Assay	2	18	2.19	0.1	4.65
Intra-Assay	3	18	2.1	0.12	5.5

Background

Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.

Protocol

These standard curves of E6640Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.426	2.43	2.394
	2.434
4ng/ml	1.323	1.349	1.314
	1.375
2ng/ml	0.706	0.661	0.625
	0.615
1ng/ml	0.419	0.355	0.32
	0.291
0.5ng/ml	0.179	0.159	0.123
	0.138
0ng/ml	0.049	0.035	0
	0.021

Gene ID	26873
UniProt ID	O14841

Research Area	Neuroscience; Cardiovascular; Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115327	E6640Hu	48T	$320		Add to Cart
BHE12115327	E6640Hu	96T	$458		Add to Cart

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