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Home / ELISA Kit

Human 5-Nucleotidase - 5-NT ELISA Kit

SKU : BHE12102507

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

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Mfr.No.

E0831Hu

Sensitivity

0.34ng/mL

Detection Range

0.5-150ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

5'-NT;5'-nucleotidase;CD antigen CD73;Ecto-5'-nucleotidase;NT5E

Molecular Weight

63368

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0831Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	23.5	1	4.2
Intra-Assay	2	18	41.2	1.41	3.4
Intra-Assay	3	18	12.2	0.8	6.5

Background

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

Protocol

These standard curves of E0831Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
80ng/mL	2.055	2.091	2.066
	2.127
40ng/mL	1.513	1.407	1.382
	1.3
20ng/mL	0.758	0.688	0.663
	0.618
10ng/mL	0.4	0.401	0.376
	0.401
5ng/mL	0.212	0.209	0.184
	0.206
0ng/mL	0.024	0.025	0
	0.026

Gene ID	4907
UniProt ID	P21589

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102507	E0831Hu	48T	$320		Add to Cart
BHE12102507	E0831Hu	96T	$458		Add to Cart

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