Human 5-NT(5-Nucleotidase) ELISA Kit

Mfr.No.

EH0468

Alternative Names

5-NT ELISA Kit, NT5E ELISA Kit, 5'-NT ELISA Kit, CD73 ELISA Kit, E5NT ELISA Kit, eNT ELISA Kit, NTE ELISA Kit, eN ELISA Kit, NT ELISA Kit, NT5 ELISA Kit, 5' nucleotidase ELISA Kit, CD73 ELISA Kit, CD73 antigen ELISA Kit, Ecto-5'-nucleotidase ELISA Kit, 5'-nucleotidase ELISA Kit, Purine 5-Prime-Nucleotidase ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

0.313-20ng/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of 5-NT and the recovery rates were calculated by comparing the measured value to the expected amount of 5-NT in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	90-103	97
EDTA plasma(n=5)	91-104	98
heparin plasma(n=5)	86-101	93

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-NT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	88-103%	94-104%	89-96%
EDTA plasma(n=5)	87-96%	82-101%	82-99%
heparin plasma(n=5)	82-100%	80-97%	80-99%

Sensitivity

< 0.188ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

5-NT/NT5E/5'-NT/CD73/E5NT/eNT/NTE/eN/NT/NT5/5' nucleotidase(CD73)/CD73 antigen/Ecto-5'-nucleotidase/5'-nucleotidase/Purine 5-Prime-Nucleotidase

Notes

For Research Use Only

UniProt ID	P21589
Antigen	5-NT

Applications	ELISA
Applications Notes	5-NT ELISA Kit allows for the in vitro quantitative determination of 5-NT concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

Reference	Journal: PLOS ONE Title: Mitochondrial function of immune cells in septic shock: A prospective observational cohort study

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SKU	Supplier No.	Size	Your price	Quantity
BHE10804939	EH0468	96T	$400		Add to Cart

Human 5-NT(5-Nucleotidase) ELISA Kit

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