Matrices listed below were spiked with certain level of 5-hydroxytryptamine and the recovery rates were calculated by comparing the measured value to the expected amount of 5-hydroxytryptamine in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
93-104
97
EDTA plasma(n=5)
87-105
95
heparin plasma(n=5)
89-102
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-hydroxytryptamine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
5-hydroxytryptamine ELISA Kit allows for the in vitro quantitative determination of 5-hydroxytryptamine concentrations in serum, plasma, tissue homogenates and other biological fluids.