Human 5-Hydroxytryptamine,5HT ELISA Kit

Mfr.No.

CSB-E08363h

Sensitivity

2 ng/mL

Detection Range

4 ng/mL-800 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

5-Hydroxytryptamine,5HT

Recovery

The recovery of mouse VEGFR-1/Flt1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-90
EDTA plasma (n=4)	108	104-112

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGFR-1/Flt1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-95
1:2	Average %	103
	Range %	100-106
1:4	Average %	85
	Range %	80-90
1:8	Average %	104
	Range %	100-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	5HT

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Essential Fatty Acids Linoleic Acid and Α‐Linolenic Acid Sex‐Dependently Regulate Glucose Homeostasis in Obesity Pan Zhuang.et al,MOL NUTR FOOD RES,June 23

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