Human 5-hydroxyeicosatetraenoic acid(5-HETE)ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E17006h

Sensitivity

7.81 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Alternative Names

5-hydroxyeicosatetraenoic acid(5-HETE)

Recovery

The recovery of human FLNA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-101
EDTA plasma (n=4)	98	90-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FLNA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	95
	Range %	91-104
1:10	Average %	89
	Range %	87-93
1:20	Average %	98
	Range %	93-102
1:40	Average %	90
	Range %	87-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	5-HETE

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Increased metabolites of 5-lipoxygenase from hypoxic ovarian cancer cells promote tumor-associated macrophage infiltration Wen Z et al,Oncogene,2014 Mar 24. Role of cyclooxygenase-mediated metabolites in lipid metabolism and expression of some immune-related genes in juvenile grass carp (Ctenopharyngodon idellus) Jing-Jing Tian.et al,Fish Physiology and Biochemistry,Dec 23

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