Human 4-HNE(4-Hydroxynonenal) ELISA Kit

Mfr.No.

EH2080

Alternative Names

4-HNE ELISA Kit, 4-Hydroxynonenal ELISA Kit, HNE ELISA Kit, 4 HNE ELISA Kit

Reactivity

Human

Detection Method

Coated with Antigen

Detection Range

31.25-2000pg/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants, Urine

Assay Type

Competitive

Recovery

Matrices listed below were spiked with certain level of 4-HNE and the recovery rates were calculated by comparing the measured value to the expected amount of 4-HNE in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	86-103	93
EDTA plasma(n=5)	90-104	97
heparin plasma(n=5)	85-101	93

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 4-HNE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	89-105%	87-102%	86-104%
EDTA plasma(n=5)	85-100%	83-95%	87-101%
heparin plasma(n=5)	81-98%	84-100%	91-99%

Sensitivity

< 18.75pg/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

4-HNE(4-Hydroxynonenal)/HNE/4 HNE

Notes

For Research Use Only

Antigen	4-HNE

Applications	ELISA
Applications Notes	4-HNE ELISA Kit allows for the in vitro quantitative determination of 4-HNE concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10800849	EH2080	96T	$400		Add to Cart

Human 4-HNE(4-Hydroxynonenal) ELISA Kit

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