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Home / ELISA Kit

Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2) ELISA Kit

SKU : BHE13702573

SRD5a2 encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH).
Steroid 5-alpha-reductase catalyzes the conversion of testosterone into the more potent androgen, dihydrotestosterone (DHT).The cDNA contains a long 3-prime untranslated region and encodes a hydrophobic 254-amino acid protein that shares 50% sequence identity with human SRD5A1 and 46% identity with rat 5-alpha-reductase. The biochemical and pharmacologic properties of the enzyme were consistent with its being the major isozyme in genital tissue.

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Mfr.No.	KTE60356
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2) ELISA Kit employs a two-site sandwich ELISA to quantitate SRD5A2.
Alternative Names	SRD5A2; MGC138457; 3-oxo-5 alpha-steroid 4-dehydrogenase 2; steroid 5-alpha-reductase 2
Detection Method	Colorimetric
Kit Components	Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2 microplate Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2 standard Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SRD5A2. No significant cross-reactivity or interference between Human SRD5A2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	SRD5a2 encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH). Steroid 5-alpha-reductase catalyzes the conversion of testosterone into the more potent androgen, dihydrotestosterone (DHT).The cDNA contains a long 3-prime untranslated region and encodes a hydrophobic 254-amino acid protein that shares 50% sequence identity with human SRD5A1 and 46% identity with rat 5-alpha-reductase. The biochemical and pharmacologic properties of the enzyme were consistent with its being the major isozyme in genital tissue.
Notes	This Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2) ELISA Kit employs a two-site sandwich ELISA to quantitate SRD5A2 in samples. An antibody specific for SRD5A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySRD5A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SRD5A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SRD5A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6716

Applications	ELISA
Applications Notes	This Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2) ELISA Kit employs a two-site sandwich ELISA to quantitate SRD5A2 in samples. An antibody specific for SRD5A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySRD5A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SRD5A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SRD5A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702573	KTE60356	48 T	$339		Add to Cart
BHE13702573	KTE60356	96 T	$589		Add to Cart
BHE13702573	KTE60356	96 Tx5	$2629		Add to Cart
BHE13702573	KTE60356	96 Tx50	$24569		Add to Cart

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