Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2(SRD5A2) ELISA kit

Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2(SRD5A2) ELISA kit

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Mfr.No.

CSB-EL022654HU

Sensitivity

6 pg/mL

Detection Range

12 pg/mL-2400 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2), MGC138457, 3-oxo-5 alpha-steroid 4-dehydrogenase 2, steroid 5-alpha-reductase 2

Recovery

The recovery of deer IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	96	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of deer IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-104
1:2	Average %	95
	Range %	89-104
1:4	Average %	99
	Range %	87-107
1:8	Average %	96
	Range %	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
200	0.175	0.174	0.175
50	0.333	0.316	0.325
16	0.622	0.640	0.631
4	1.040	1.048	1.044
1	1.416	1.389	1.403
0	2.577	2.633	2.605

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P31213

Applications	ELISA
Research Area	SRD5A2

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10500995	CSB-EL022654HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.