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Home / ELISA Kit

Human 3-Hydroxyanthranilate 3 - 4-dioxygenase - HAAO ELISA Kit

SKU : BHE12106152

Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.

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Mfr.No.

E4879Hu

Sensitivity

9.81ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

3-HAO;3-hydroxyanthranilate 3,4-dioxygenase;3-hydroxyanthranilate oxygenase;3-hydroxyanthranilic acid dioxygenase;h3HAO;HAAO;HAD;HAO;VCRL1;

Molecular Weight

32556

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E4879Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1163.17	44.25	3.8
Intra-Assay	2	18	1415.91	34.77	2.46
Intra-Assay	3	18	777.07	29.31	3.77

Background

Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.

Protocol

These standard curves of E4879Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.058	1.956	1.906
	1.853
1200ng/L	1.128	1.112	1.062
	1.095
600ng/L	0.384	0.441	0.392
	0.498
300ng/L	0.229	0.274	0.224
	0.318
150ng/L	0.122	0.13	0.081
	0.138
0ng/L	0.05	0.049	0
	0.049

Gene ID	23498
UniProt ID	P46952

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106152	E4879Hu	48T	$320		Add to Cart
BHE12106152	E4879Hu	96T	$458		Add to Cart

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