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Home / ELISA Kit

Human 28S ribosomal protein S36, mitochondrial (MRPS36) ELISA Kit

SKU : BHE13703740

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. The mitochondrial ribosome (mitoribosome) consists of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPS36 encodes a 28S subunit protein. Pseudogenes corresponding to this gene are found on chromosomes 3p, 4q, 8p, 11q, 12q, and 20p.

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Mfr.No.	KTE61525
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human 28S ribosomal protein S36, mitochondrial (MRPS36) ELISA Kit employs a two-site sandwich ELISA to quantitate MRPS36.
Alternative Names	MRPS36; DC47; MGC22896; MRP-S36;
Detection Method	Colorimetric
Kit Components	Human 28S ribosomal protein S36, mitochondrial microplate Human 28S ribosomal protein S36, mitochondrial standard Human 28S ribosomal protein S36, mitochondrial detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human 28S ribosomal protein S36, mitochondrial (MRPS36) ELISA Kit has high sensitivity and excellent specificity for detection of Human MRPS36. No significant cross-reactivity or interference between Human MRPS36 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. The mitochondrial ribosome (mitoribosome) consists of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPS36 encodes a 28S subunit protein. Pseudogenes corresponding to this gene are found on chromosomes 3p, 4q, 8p, 11q, 12q, and 20p.
Notes	This Human 28S ribosomal protein S36, mitochondrial (MRPS36) ELISA Kit employs a two-site sandwich ELISA to quantitate MRPS36 in samples. An antibody specific for MRPS36 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRPS36 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRPS36 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRPS36 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	92259

Applications	ELISA
Applications Notes	This Human 28S ribosomal protein S36, mitochondrial (MRPS36) ELISA Kit employs a two-site sandwich ELISA to quantitate MRPS36 in samples. An antibody specific for MRPS36 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRPS36 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRPS36 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRPS36 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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