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Home / ELISA Kit

Human 26S proteasome non-ATPase regulatory subunit 10 (PSMD10) ELISA Kit

SKU : BHE13703297

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.
PSMD10 encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.The predicted 226-amino acid human p28 protein contains 5 ankyrin repeats, which are thought to function in protein-protein interactions.

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Mfr.No.	KTE61081
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human 26S proteasome non-ATPase regulatory subunit 10 (PSMD10) ELISA Kit employs a two-site sandwich ELISA to quantitate PSMD10.
Alternative Names	PSMD10; RP5-889N15.3; dJ889N15.2; p28; 26S proteasome non-ATPase regulatory subunit 10; 26S proteasome regulatory subunit p28; ankyrin repeat protein; gankyrin; hepatocellular carcinoma-associated protein p28-I
Detection Method	Colorimetric
Kit Components	Human 26S proteasome non-ATPase regulatory subunit 10 microplate Human 26S proteasome non-ATPase regulatory subunit 10 standard Human 26S proteasome non-ATPase regulatory subunit 10 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human 26S proteasome non-ATPase regulatory subunit 10 (PSMD10) ELISA Kit has high sensitivity and excellent specificity for detection of Human PSMD10. No significant cross-reactivity or interference between Human PSMD10 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMD10 encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.The predicted 226-amino acid human p28 protein contains 5 ankyrin repeats, which are thought to function in protein-protein interactions.
Notes	This Human 26S proteasome non-ATPase regulatory subunit 10 (PSMD10) ELISA Kit employs a two-site sandwich ELISA to quantitate PSMD10 in samples. An antibody specific for PSMD10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSMD10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSMD10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSMD10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5716

Applications	ELISA
Applications Notes	This Human 26S proteasome non-ATPase regulatory subunit 10 (PSMD10) ELISA Kit employs a two-site sandwich ELISA to quantitate PSMD10 in samples. An antibody specific for PSMD10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSMD10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSMD10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSMD10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703297	KTE61081	48 T	$339		Add to Cart
BHE13703297	KTE61081	96 T	$589		Add to Cart
BHE13703297	KTE61081	96 Tx5	$2629		Add to Cart
BHE13703297	KTE61081	96 Tx50	$24569		Add to Cart

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