Human 2-oxoisovalerate Dehydrogenase Subunit Alpha - Mitochondrial

Mfr.No.

E4421Hu

Sensitivity

0.021ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human 2-Oxoisovalerate Dehydrogenase Subunit Alpha, Mitochondrial (also known as BCKDHA) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial;BCKDE1A;BCKDH E1-alpha;BCKDHA;Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain;MSU;MSUD1;OVD1A

Molecular Weight

50471

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E4421Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.04	0.13	4.44
Intra-Assay	2	18	3.97	0.11	2.87
Intra-Assay	3	18	7.54	0.26	3.41

Background

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).

Protocol

These standard curves of E4421Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.003	2.141	2.107
	2.278
6ng/ml	1.524	1.453	1.42
	1.383
3ng/ml	0.692	0.673	0.639
	0.655
1.5ng/ml	0.307	0.319	0.285
	0.332
0.75ng/ml	0.165	0.187	0.153
	0.209
0ng/ml	0.036	0.034	0
	0.031

Gene ID	593
UniProt ID	P12694

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105771	E4421Hu	48T	$320		Add to Cart
BHE12105771	E4421Hu	96T	$458		Add to Cart

Human 2-oxoisovalerate Dehydrogenase Subunit Alpha - Mitochondrial - BCKDHA ELISA Kit

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