Human 2,3-Diphosphoglycerate,2,3-DPG ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09861h

Sensitivity

0.078 umol/mL

Detection Range

0.312 umol/mL-20 umol/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

2,3-Diphosphoglycerate,2,3-DPG

Recovery

The recovery of mouse FLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	101	95-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	97
	Range %	92-101
1:4	Average %	88
	Range %	86-95
1:8	Average %	90
	Range %	86-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	2,3DPG

Storage	Store at 2-8°C. Please refer to protocol.

Reference	In vitro efficacy of frozen erythrocytes: implementation of new strategic blood stores to alleviate resource shortage (issue revisited). Cetinkaya RA.et al,Turk J Med Sci.,45(3):638-43. Temporary impact of blood donation on physical performance and hematologic variables in women Stangerup I.et al,Transfusion. ,28 April

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