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Home / ELISA Kit

Human 182 kDa tankyrase-1-binding protein (TNKS1BP1) ELISA Kit

SKU : BHE13702413

TNKS1BP1, has a calculated molecular mass of 182 kD. It is predicted to contain 2 basic regions at its N and C termini and a large internal acidic region. The C terminus also contains 2 potential nuclear localization signals. Northern blot analysis detected transcripts of 7, 4.4, and 2.3 kb in multiple tissues, with highest expression in testis, ovary, and lung, and minimal expression in brain and peripheral blood leukocytes. By immunolocalization of endogenous TAB182 in 2 human cell lines, Seimiya and Smith (2002) found TAB182 in the cytoplasm, where it colocalized with cortical actin, and in the nucleus. The nuclear staining was similar to that found for heterochromatin proteins. In a synchronized population of HeLa cells, TAB182 associated with chromosomes throughout mitosis.

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Mfr.No.	KTE60194
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human 182 kDa tankyrase-1-binding protein (TNKS1BP1) ELISA Kit employs a two-site sandwich ELISA to quantitate TNKS1BP1.
Alternative Names	TNKS1BP1; FLJ45975; KIAA1741; TAB182; tankyrase 1-binding protein 1; tankyrase 1-binding protein of 182 kDa
Detection Method	Colorimetric
Kit Components	Human 182 kDa tankyrase-1-binding protein microplate Human 182 kDa tankyrase-1-binding protein standard Human 182 kDa tankyrase-1-binding protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human 182 kDa tankyrase-1-binding protein (TNKS1BP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNKS1BP1. No significant cross-reactivity or interference between Human TNKS1BP1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TNKS1BP1, has a calculated molecular mass of 182 kD. It is predicted to contain 2 basic regions at its N and C termini and a large inteal acidic region. The C terminus also contains 2 potential nuclear localization signals. Northe blot analysis detected transcripts of 7, 4.4, and 2.3 kb in multiple tissues, with highest expression in testis, ovary, and lung, and minimal expression in brain and peripheral blood leukocytes. By immunolocalization of endogenous TAB182 in 2 human cell lines, Seimiya and Smith (2002) found TAB182 in the cytoplasm, where it colocalized with cortical actin, and in the nucleus. The nuclear staining was similar to that found for heterochromatin proteins. In a synchronized population of HeLa cells, TAB182 associated with chromosomes throughout mitosis.
Notes	This Human 182 kDa tankyrase-1-binding protein (TNKS1BP1) ELISA Kit employs a two-site sandwich ELISA to quantitate TNKS1BP1 in samples. An antibody specific for TNKS1BP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNKS1BP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNKS1BP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNKS1BP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	85456

Applications	ELISA
Applications Notes	This Human 182 kDa tankyrase-1-binding protein (TNKS1BP1) ELISA Kit employs a two-site sandwich ELISA to quantitate TNKS1BP1 in samples. An antibody specific for TNKS1BP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNKS1BP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNKS1BP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNKS1BP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702413	KTE60194	48 T	$339		Add to Cart
BHE13702413	KTE60194	96 T	$589		Add to Cart
BHE13702413	KTE60194	96 Tx5	$2629		Add to Cart
BHE13702413	KTE60194	96 Tx50	$24569		Add to Cart

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