Matrices listed below were spiked with certain level of 15-LO and the recovery rates were calculated by comparing the measured value to the expected amount of 15-LO in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-94
90
EDTA plasma(n=5)
88-105
95
heparin plasma(n=5)
94-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 15-LO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
15-LO ELISA Kit allows for the in vitro quantitative determination of 15-LO concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human 15-LO(Arachidonate 15-Lipoxygenase) ELISA Kit