Matrices listed below were spiked with certain level of 15-HETE and the recovery rates were calculated by comparing the measured value to the expected amount of 15-HETE in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-102
96
EDTA plasma(n=5)
85-105
95
heparin plasma(n=5)
90-101
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 15-HETE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-105%
89-103%
86-99%
EDTA plasma(n=5)
82-97%
86-101%
87-100%
heparin plasma(n=5)
81-100%
80-93%
82-88%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
15-HETE ELISA Kit allows for the in vitro quantitative determination of 15-HETE concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human 15-HETE (15-Hydroxyeicosatetraenoic Acid) ELISA Kit