Human 14-3-3 protein zeta/delta(YWHAZ) ELISA kit

Human 14-3-3 protein zeta/delta(YWHAZ) ELISA kit

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Mfr.No.

CSB-EL026293HU

Alternative Names

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide, 14-3-3-zeta, KCIP-1, MGC111427, MGC126532, MGC138156, 14-3-3 protein/cytosolic phospholipase A2, 14-3-3 zeta, OTTHUMP00000165851, OTTHUMP00000165858, phospholipase A2, protein kinase C inhibitor protein-

Reactivity

Human

Detection Range

0.625 ng/mL-40 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human HIST1H4A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human HIST1H4A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	101
	Range %	95-108
1:2	Average %	88
	Range %	83-99
1:4	Average %	89
	Range %	87-92
1:8	Average %	110
	Range %	106-115

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2400	2.757	2.657	2.707	2.539
1200	2.024	1.924	1.974	1.806
600	1.226	1.146	1.186	1.018
300	0.665	0.655	0.660	0.492
150	0.390	0.380	0.385	0.217
75	0.281	0.271	0.276	0.108
37.5	0.225	0.215	0.220	0.052
0	0.173	0.163	0.168	?

Species

Homo sapiens

Sensitivity

0.156 ng/mL

Limit Of Detection

450 nm

UniProt ID	P63104
Antigen	tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide

Applications

ELISA

Research Area

YWHAZ

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500976	CSB-EL026293HU	96T	$695	Add to Cart
BHE10500976	CSB-EL026293HU	5x96T	$3336	Add to Cart
BHE10500976	CSB-EL026293HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.