Human 14-3-3 Protein Theta - YWHAQ ELISA Kit

Mfr.No.

E4869Hu

Sensitivity

0.091ng/ml

Detection Range

0.2-60ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

14-3-3 protein tau;14-3-3 protein T-cell;14-3-3 protein theta;Protein HS1;YWHAQ

Molecular Weight

27764

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E4869Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	14.7	0.43	2.9
Intra-Assay	2	18	10.3	0.58	5.6
Intra-Assay	3	18	6.6	0.42	6.3

Background

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.

Protocol

These standard curves of E4869Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/ml	2.111	2.174	2.119
	2.237
16ng/ml	1.332	1.399	1.344
	1.466
8ng/ml	0.88	0.821	0.766
	0.762
4ng/ml	0.52	0.497	0.442
	0.473
2ng/ml	0.3	0.276	0.221
	0.251
0ng/ml	0.039	0.055	0
	0.071

Gene ID	10971
UniProt ID	P27348

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106142	E4869Hu	48T	$320		Add to Cart
BHE12106142	E4869Hu	96T	$458		Add to Cart

Human 14-3-3 Protein Theta - YWHAQ ELISA Kit

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