Human 14-3-3 protein sigma(SFN) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL021135HU

Sensitivity

0.27 ng/ml

Detection Range

0.27 ng/ml- 200ng/ml

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Recovery

The recovery of bovine IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	97	92-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-99
1:200	Average %	97
	Range %	92-103
1:400	Average %	85
	Range %	81-87
1:800	Average %	89
	Range %	83-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

UniProt ID	P31947

Applications	ELISA
Research Area	SFN

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500974	CSB-EL021135HU	96T	$695	Add to Cart
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BHE10500974	CSB-EL021135HU	10x96T	$6602.50	Add to Cart