Human 14-3-3 Protein Gamma - YWHAG ELISA Kit

Mfr.No.

E3752Hu

Sensitivity

0.16ng/ml

Detection Range

0.3-90ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

14-3-3 protein gamma;14-3-3 protein gamma, N-terminally processed;KCIP-1;Protein kinase C inhibitor protein 1;YWHAG

Molecular Weight

28303

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3752Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	7	0.48	6.9
Intra-Assay	2	18	20.9	1.24	5.9
Intra-Assay	3	18	24.9	0.68	2.7

Background

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Protocol

These standard curves of E3752Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/ml	2.139	2.245	2.214
	2.351
24ng/ml	1.284	1.223	1.193
	1.163
12ng/ml	0.795	0.736	0.705
	0.676
6ng/ml	0.531	0.516	0.485
	0.5
3ng/ml	0.254	0.226	0.196
	0.199
0ng/ml	0.033	0.03	0
	0.027

Gene ID	7532
UniProt ID	P61981

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105213	E3752Hu	48T	$320		Add to Cart
BHE12105213	E3752Hu	96T	$458		Add to Cart

Human 14-3-3 Protein Gamma - YWHAG ELISA Kit

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