Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of 14-3-3 protein eta and the recovery rates were calculated by comparing the measured value to the expected amount of 14-3-3 protein eta in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-101
91
EDTA plasma(n=5)
90-105
99
heparin plasma(n=5)
89-98
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 14-3-3 protein eta and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
14-3-3 protein eta ELISA Kit allows for the in vitro quantitative determination of 14-3-3 protein eta concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human 14-3-3 protein eta (YWHAH) ELISA Kit