Human 14-3-3 protein eta(YWHAH) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL026289HU

Alternative Names

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide, LL22NC03-44A4.1, YWHA1, 14-3-3 eta

Reactivity

Human

Detection Range

0.625 ng/mL-40 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat HIF-1? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-95
EDTA plasma (n=4)	95	88-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat HIF-1? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-94
1:2	Average %	96
	Range %	91-100
1:4	Average %	93
	Range %	87-97
1:8	Average %	100
	Range %	96-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.156 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q04917
Antigen	tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide

Applications

ELISA

Research Area

YWHAH

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10500972	CSB-EL026289HU	96T	$695	Add to Cart
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