Matrices listed below were spiked with certain level of 10-HETE and the recovery rates were calculated by comparing the measured value to the expected amount of 10-HETE in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
97-102
100
EDTA plasma(n=5)
90-105
97
heparin plasma(n=5)
87-105
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 10-HETE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-104%
86-92%
86-94%
EDTA plasma(n=5)
85-100%
84-99%
86-93%
heparin plasma(n=5)
81-95%
81-95%
82-96%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
10-HETE ELISA Kit allows for the in vitro quantitative determination of 10-HETE concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human 10-HETE(10-Hydroxyeicosatetraenoic Acid) ELISA Kit