Human 1-Phosphatidylinositol-4 - 5-bisphosphate Phosphodiesterase Beta-3

Mfr.No.

E4872Hu

Sensitivity

9.65ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3;Phosphoinositide phospholipase C-beta-3;Phospholipase C-beta-3;PLCB 3;PLCB3;PLC-beta-3

Molecular Weight

138799

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E4872Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1236.2	54.33	4.4
Intra-Assay	2	18	2111.1	67.26	3.2
Intra-Assay	3	18	678.4	27.12	4

Background

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

Protocol

These standard curves of E4872Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	1.948	1.986	1.939
	2.023
1200ng/L	1.093	1.047	1
	1.001
600ng/L	0.391	0.4	0.352
	0.408
300ng/L	0.182	0.184	0.136
	0.185
150ng/L	0.113	0.126	0.079
	0.138
0ng/L	0.063	0.047	0
	0.032

Gene ID	5331
UniProt ID	Q01970

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106145	E4872Hu	48T	$320		Add to Cart
BHE12106145	E4872Hu	96T	$458		Add to Cart

Human 1-Phosphatidylinositol-4 - 5-bisphosphate Phosphodiesterase Beta-3 - PLCB3 ELISA Kit

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