HS Human IL-6 (Interleukin 6) ELISA Kit

Mfr.No.

E-HSEL-H0003

Sensitivity

0.49 pg/mL

Detection Range

0.78-50 pg/mL

Reactivity

Human

Assay Duration

3.5h

Assay Type

Sandwich

Description

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-6. You can calculate the concentration of Human IL-6 in the samples by comparing the OD of the samples to the standard curve.

Detection Method

Colormetric

Kit Components

An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells x12 strips 48T: 8 wells x6 strips	-20°C, 12 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100x)	96T: 1 vial, 120 uL 48T: 1 vial, 60 uL
Concentrated HRP Conjugate (100x)	96T: 1 vial, 120 uL 48T: 1 vial, 60 uL	-20°C(Protect from light), 12 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 12 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25x)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8°C(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Recovery

80%-120%. The recovery of Human IL-6 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	85-99	91
EDTA plasma (n=8)	89-104	95
Cell culture media (n=8)	96-111	102

Specificity

This kit recognizes Human IL-6 in samples. No significant cross-reactivity or interference between Human IL-6 and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-6 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-6 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	2.71	7.51	18.99	2.93	6.94	18.87
Standard deviation	0.16	0.31	0.71	0.19	0.39	0.62
CV (%)	5.82	4.10	3.76	6.38	5.58	3.29

Usage Notes

This ELISA kit applies to the in vitro quantitative determination of Human IL-6 concentrations in serum, plasma and other biological fluids.

UniProt ID	P05231

Applications	ELISA
Applications Notes	This ELISA kit applies to the in vitro quantitative determination of Human IL-6 concentrations in serum, plasma and other biological fluids.
Research Area	Cancer, Metabolism, Immunology, Neuroscience

Storage	2-8°C/-20°C,12 months
Shipping	Ice bag

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SKU	Supplier No.	Size	Your price	Quantity
BHE10308892	E-HSEL-H0003	96T	$520		Add to Cart
BHE10308892	E-HSEL-H0003	48T	$416		Add to Cart

HS Human IL-6 (Interleukin 6) ELISA Kit

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