Matrices listed below were spiked with certain level of HS and the recovery rates were calculated by comparing the measured value to the expected amount of HS in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-103
96
EDTA plasma(n=5)
92-104
98
heparin plasma(n=5)
90-101
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
95-99%
98-105%
97-105%
EDTA plasma(n=5)
93-101%
93-101%
89-101%
heparin plasma(n=5)
95-99%
94-100%
98-100%
Sensitivity
< 46.9ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
HS ELISA Kit allows for the in vitro quantitative determination of HS concentrations in serum, plasma, tissue homogenates and other biological fluids.