Horse thyroxine (T4) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EQ027512HO

Sensitivity

10 ng/mL

Detection Range

20 ng/mL-320 ng/mL

Reactivity

Horse

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

thyroxine (T4)

Recovery

The recovery of mouse Hcy spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	95	91-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hcy in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-105
1:2	Average %	90
	Range %	86-96
1:4	Average %	94
	Range %	90-98
1:8	Average %	99
	Range %	95-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Equus caballus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	T4

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10500960	CSB-EQ027512HO	96T	$495	Add to Cart
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BHE10500960	CSB-EQ027512HO	10x96T	$4702.50	Add to Cart