Matrices listed below were spiked with certain level of MSTN and the recovery rates were calculated by comparing the measured value to the expected amount of MSTN in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-100
93
EDTA plasma(n=5)
86-100
92
heparin plasma(n=5)
86-105
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MSTN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
91-105%
89-104%
85-103%
EDTA plasma(n=5)
82-97%
86-99%
85-98%
heparin plasma(n=5)
86-93%
87-99%
83-98%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
MSTN ELISA Kit allows for the in vitro quantitative determination of MSTN concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Horse MSTN(MSTN/Growth differentiation factor 8) ELISA Kit