Matrices listed below were spiked with certain level of MCP-1 and the recovery rates were calculated by comparing the measured value to the expected amount of MCP-1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-101
92
EDTA plasma(n=5)
87-104
92
heparin plasma(n=5)
87-98
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MCP-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-103%
88-100%
86-104%
EDTA plasma(n=5)
83-96%
82-97%
82-96%
heparin plasma(n=5)
83-95%
85-100%
84-92%
Sensitivity
< 0.938pg/ml
Notes
For Research Use Only
UniProt ID
Q9TTQ3
Applications
ELISA
Applications Notes
MCP-1 ELISA Kit allows for the in vitro quantitative determination of MCP-1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Horse MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit