Horse Interleukin-12 Subunit Alpha

Mfr.No.

E0129HO

Alternative Names

CLMF p35;Cytotoxic lymphocyte maturation factor 35 kDa subunit;IL 12A;IL 12alpha;IL-12 subunit p35;IL12A;IL-12A;Interleukin-12 subunit alpha

Reactivity

Horse

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

24800

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.78ng/L

Background

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.

Gene ID	100034215
UniProt ID	Q9XSQ6
Antigen	IL12A

Research Area

Others

Protocol

These standard curves of E0129HO are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.021	1.999	1.955
	1.976
1200ng/L	1.543	1.54	1.497
	1.537
600ng/L	0.75	0.75	0.706
	0.749
300ng/L	0.457	0.393	0.35
	0.33
150ng/L	0.196	0.192	0.148
	0.187
0ng/L	0.03	0.043	0
	0.057

Precision

We measured random samples of E0129HO within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	601.2	22.24	3.7
Intra-Assay	2	18	1715.9	118.51	6.9
Intra-Assay	3	18	306.5	10.79	3.5

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101737	E0129HO	96T	$498		Add to Cart
BHE12101737	E0129HO	48T	$360		Add to Cart

Horse Interleukin-12 Subunit Alpha - IL12A ELISA Kit

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