Horse hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A) ELISA kit

Mfr.No.

CSB-EL010351HO

Sensitivity

7.81 pg/mL

Reactivity

Horse

Sample Type

Serum, Tissue homogenates, Plasma

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A)

Recovery

The recovery of mouse Cyt-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	95-105
EDTA plasma (n=4)	87	83-91

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Cyt-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-97
1:2	Average %	84
	Range %	80-88
1:4	Average %	90
	Range %	85-94
1:8	Average %	97
	Range %	92-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Equus caballus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	F6VG41
Antigen	hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A)

Applications	ELISA
Research Area	HIF1A

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10500917	CSB-EL010351HO	10x96T	$7505	Add to Cart