Human Fibroblast Growth Factor 2, Basic (FGF2) (Competitive EIA) ELISA Kit

Mfr.No.

HEA551Hu

Sensitivity

0.87pg/mL

Detection Range

2.47-200pg/mL

Reactivity

Human

Assay Duration

Assay Type

Competitive Inhibition

Sample Volume

50 ul(Please refer to the manual for the specific data.)

Description

This assay has high sensitivity and excellent specificity for detection of High Sensitive Fibroblast Growth Factor 2, Basic (FGF2).
No significant cross-reactivity or interference between High Sensitive Fibroblast Growth Factor 2, Basic (FGF2) and analogues was observed.

Alternative Names

B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2

Detection Method

Colormetric

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
Reagent Diluent	1×300µL	Stop Solution	1×6mL
TMB Substrate	1×9mL	Instruction manual	1
Wash Buffer (30 × concentrate)	1×20mL

Recovery

Matrices listed below were spiked with certain level of recombinant High Sensitive Fibroblast Growth Factor 2, Basic (FGF2) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Fibroblast Growth Factor 2, Basic (FGF2) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	96-105	101
EDTA plasma(n=5)	95-102	99
heparin plasma(n=5)	81-105	93

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Fibroblast Growth Factor 2, Basic (FGF2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	89-104%	97-105%	88-102%	88-102%
EDTA plasma(n=5)	85-94%	79-101%	95-103%	79-101%
heparin plasma(n=5)	84-92%	84-94%	82-99%	98-105%

Features

Detects protein (low-target protein, high-sensitivity)

Species

Human

Sample Type

Cell lysates, Tissue homogenates, Other biological fluids

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Fibroblast Growth Factor 2, Basic (FGF2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Fibroblast Growth Factor 2, Basic (FGF2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: C<10%
Inter-Assay: C<12%

Notes

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Fibroblast Growth Factor 2, Basic (FGF2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Fibroblast Growth Factor 2, Basic (FGF2) and unlabeled High Sensitive Fibroblast Growth Factor 2, Basic (FGF2) (Standards or samples) with the pre-coated antibody specific to High Sensitive Fibroblast Growth Factor 2, Basic (FGF2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Fibroblast Growth Factor 2, Basic (FGF2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Fibroblast Growth Factor 2, Basic (FGF2) in the sample.

Usage Notes

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Gene ID	2247
UniProt ID	P09038
Tissue Specificity	Reactive with Human FGF2 / Fibroblast Growth Factor 2, Basic

Applications	ELISA
Research Area	Cytokine

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

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SKU	Supplier No.	Size	Your price
BHE10109750	HEA551Hu	96T*1	$515	Add to Cart
BHE10109750	HEA551Hu	96T*5	$2317.50	Add to Cart
BHE10109750	HEA551Hu	96T*10	$4377.50	Add to Cart

High Sensitive ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2) (Human)

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