Rat Cardiac Troponin I (cTnI) (Sandwich ELISA) ELISA Kit

Mfr.No.

HEA478Ra

Sensitivity

1.29pg/mL

Detection Range

3.12-200pg/mL

Reactivity

Rat

Sample Type

Plasma

Assay Duration

Assay Type

Sandwich

Description

This assay has high sensitivity and excellent specificity for detection of High Sensitive Cardiac Troponin I (cTnI).
No significant cross-reactivity or interference between High Sensitive Cardiac Troponin I (cTnI) and analogues was observed.

Alternative Names

TNNI3; TNNC1; CMH7; C-TnI; Troponin I Type 3; Troponin I Type 3, Cardiac

Detection Method

Colormetric

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
TMB Substrate	1×9mL	Stop Solution	1×6mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Recovery

Matrices listed below were spiked with certain level of recombinant High Sensitive Cardiac Troponin I (cTnI) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Cardiac Troponin I (cTnI) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	90-97	93
EDTA plasma(n=5)	86-101	90
heparin plasma(n=5)	87-98	94

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Cardiac Troponin I (cTnI) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	78-99%	96-103%	91-99%	93-102%
EDTA plasma(n=5)	99-105%	98-105%	79-98%	80-101%
heparin plasma(n=5)	98-105%	94-101%	97-104%	80-96%

Features

Detects protein (low-target protein, high-sensitivity)

Species

Rat

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Cardiac Troponin I (cTnI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Cardiac Troponin I (cTnI) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: C<10%
Inter-Assay: C<12%

Notes

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Cardiac Troponin I (cTnI) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Cardiac Troponin I (cTnI) and unlabeled High Sensitive Cardiac Troponin I (cTnI) (Standards or samples) with the pre-coated antibody specific to High Sensitive Cardiac Troponin I (cTnI). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Cardiac Troponin I (cTnI) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Cardiac Troponin I (cTnI) in the sample.

Usage Notes

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Gene ID	29248
UniProt ID	P23693
Antigen	cTnI

Applications	ELISA
Research Area	Cardiovascular biology

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

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SKU	Supplier No.	Size	Your price
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High Sensitive ELISA Kit for Cardiac Troponin I (cTnI) (Rat)

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