TIMP-2 ELISA Kit, Tissue Inhibitors of Metalloproteinase 2 ELISA Kit
Reactivity
Hamster
Detection Method
Double Antibody
Detection Range
0.156-10ng/ml
Sample Type
Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of TIMP-2 and the recovery rates were calculated by comparing the measured value to the expected amount of TIMP-2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-103
97
EDTA plasma(n=5)
86-101
92
heparin plasma(n=5)
89-101
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TIMP-2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-102%
91-105%
87-98%
EDTA plasma(n=5)
85-101%
88-93%
85-93%
heparin plasma(n=5)
86-97%
87-98%
87-99%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
TIMP-2 ELISA Kit allows for the in vitro quantitative determination of TIMP-2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Hamster TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) ELISA Kit