Hamster Interleukin 6,IL-6 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14304Ha

Sensitivity

1.95 pg/mL

Detection Range

7.8 pg/mL-500 pg/mL

Reactivity

Golden hamster

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Interleukin 6,IL-6

Recovery

The recovery of human LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	86-94
EDTA plasma (n=4)	101	97-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human LIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-97
1:2	Average %	103
	Range %	98-106
1:4	Average %	87
	Range %	83-91
1:8	Average %	98
	Range %	92-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mesocricetus auratus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	IL-6

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Effects of combined extract of cocoa, coffee, green tea and garcinia on lipid profiles, glycaemicMarkers and inflammatory responses in hamsters Chang CW. et al,BMC Complement Altern Med,2015 Aug 12;15:269. Effect of a single point mutation on equine herpes virus 9 (EHV-9) neuropathogenicity after intranasal inoculation in a hamster model Saleh AG.et al,J Vet Med Sci.,Jul 15 Clinical effect of three important cytokines on the pathogenesis of equine herpes virus 9 (EHV-9), equine herpes virus 1 (EHV-1) and Zebra borne EHV-1 Asmaa G. Saleh.et al,IOSR Journal of Agriculture and Veterinary Science,/

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