Hamster Interleukin 1beta,IL-1beta ELISA Kit

Mfr.No.

CSB-E14259Ha

Sensitivity

3.12 pg/mL

Detection Range

12.5 pg/mL-800 pg/mL

Reactivity

Golden hamster

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Interleukin 1beta,IL-1beta, IL-1beta,Interleukin 1beta

Recovery

The recovery of human LGALS8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	81-93
EDTA plasma (n=4)	95	88-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human LGALS8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	87-99
1:2	Average %	87
	Range %	83-92
1:4	Average %	90
	Range %	84-93
1:8	Average %	99
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mesocricetus auratus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q9WVQ9

Applications	ELISA
Research Area	IL1B

Storage	Store at 2-8°C. Please refer to protocol.

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