Matrices listed below were spiked with certain level of ADP/Acrp30 and the recovery rates were calculated by comparing the measured value to the expected amount of ADP/Acrp30 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-104
96
EDTA plasma(n=5)
93-105
100
heparin plasma(n=5)
91-98
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ADP/Acrp30 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-97%
87-102%
86-103%
EDTA plasma(n=5)
87-99%
85-99%
83-99%
heparin plasma(n=5)
80-98%
87-96%
85-97%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
ADP/Acrp30 ELISA Kit allows for the in vitro quantitative determination of ADP/Acrp30 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Hamster ADP/Acrp30 (Adiponectin) ELISA Kit