Matrices listed below were spiked with certain level of OVA sIgE and the recovery rates were calculated by comparing the measured value to the expected amount of OVA sIgE in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
84-100
92
EDTA plasma(n=5)
82-103
93
heparin plasma(n=5)
81-103
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OVA sIgE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-102%
86-101%
80-95%
EDTA plasma(n=5)
91-97%
91-101%
94-101%
heparin plasma(n=5)
93-99%
92-98%
90-100%
Sensitivity
< 0.938ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
Guinea pig OVA sIgE ELISA Kit allows for the in vitro quantitative determination of OVA sIgE concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Guinea pig OVA sIgE (Ovalbumin Specific IgE) ELISA Kit