Guinea pig interleukin 5,IL-5 ELISA Kit

Guinea pig interleukin 5,IL-5 ELISA Kit

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Mfr.No.

CSB-E12064Gp

Sensitivity

7.81 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Guinea pig

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

interleukin 5 (colony-stimulating factor, eosinophil), EDF, IL-5, TRF, B cell differentiation factor I, T-cell replacing factor, eosinophil differentiation factor, interleukin 5, interleukin-5

Recovery

The recovery of human resistin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-104
EDTA plasma (n=4)	92	88-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human resistin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-103
1:2	Average %	94
	Range %	91-98
1:4	Average %	102
	Range %	98-106
1:8	Average %	86
	Range %	80-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.285	2.330	2.308	2.204
10	1.350	1.427	1.389	1.285
5	0.701	0.733	0.717	0.613
2.5	0.400	0.424	0.412	0.308
1.25	0.256	0.268	0.262	0.158
0.625	0.178	0.169	0.174	0.070
0.312	0.132	0.129	0.131	0.027
0	0.105	0.102	0.104	?

Species

Cavia porcellus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O08987

Applications	ELISA
Research Area	IL5

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500845	CSB-E12064Gp	96T	$615	Add to Cart
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BHE10500845	CSB-E12064Gp	10x96T	$5842.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.